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KMID : 0364819710090030095
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Korean Journal of Microbiology
1971 Volume.9 No. 3 p.95 ~ p.102
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Production of Single-Cell Protein on Petroleum Hydrocarbon (III); On the Growth of Candida tropicalis KIST 351
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Abstract
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The growth characteristics of Candida tropicalis KIST 351 on gas oil substrate under different culture conditions were investigated and the preliminary animal feeding experiments using this yeast as a partial substitute of fish meal was also conducted. The yeast assimilates effectively n-paraffins in gas oil ranging from C_16 to C_18 with its maximum cell growth at 33℃ and pH 5.5 with aeration of 3 vvn and agitation of 900 rpm. The optimal concentrations of nitrogen sources, KH₂PO₄and Na₂HPO₄were 4, 2 and 0.5g/1, respectively. Ferrous sulfate, manganese sulfate and zinc sulfate showed positive effect to cell growth with the optimal range of 5-10 ppm. In the feeding experiment with 3 and 5% incorporation of the gas oil grown yeast, neither adverse effects on growth of chicks nor toxic effect were observed. Protein content of the dried cell was 58.8% and its amino acid composition compared well with other single-cell protein products and FAO reference protein.
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KEYWORD
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